Active Recombinant SARS Spike Protein S1 Domain, Fc-tagged

• Cat.No.: Spike-43V
• Product Overview: Recombinant SARS Spike Protein S1 Domain (19-713aa) was fused to Fc-tag and expressed using recombinant baculoviruses and purified from the infected cell supernatant by protein A affinity chromatography.

Specification

• Species: SARS-CoV
• Source: Baculovirus
• Tag: Fc
• Protein Length: 19-713aa
• Description: Spike protein S1 attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. Binding to human ACE2 and CLEC4M/DC-SIGNR receptors and internalization of the virus into the endosomes of the host cell induces conformational changes in the S glycoprotein. Proteolysis by cathepsin CTSL may unmask the fusion peptide of S2 and activate membranes fusion within endosomes.
• Form: Freeze-dried in PBS without preservative.
• Bio-activity: Purified SCoV S1-Fc is active in binding the Vero E6 cell surface and recombinant ACE2.
• Purity: > 90%
• Storage: Desiccated at +2 to +8 centigrade. Reconstituted product should be stored in aliquots at –20 centigrade.
• Reconstitution: Reconstitute with sterile double distilled water. Recommended final concentration 1 mg/ml.

Gene Information

• Gene Name: S
• Official Symbol: S
• Synonyms: E2
• Gene ID: 1489668
• Protein Refseq: NP_828851.1
• UniProt ID: P59594