Active Recombinant Vaccinia Virus B19R, His-tagged

• Cat.No.: B19R-190V
• Product Overview: Vaccinia Virus B18R Protein, His 20 - Glu 351 (Accession # P25213), was produced in human 293 cells (HEK293).

Specification

• Species: VACV
• Source: HEK293
• Tag: His
• Protein Length: 20-351 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% Trehalose.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized human IFN-α1a at 2μg/mL (100 µL/well),can bind Vaccinia Virus B18R, His Tag with a linear range of 0.8-10 ng/mL.
• Molecular Mass: Vaccinia Virus B18R, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 40.3 kDa. The predicted N-terminus is His 20. The reducing (R) protein migrates as 50-60 kDa in SDS-PAGE due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: B19R temporal expression: early [ Vaccinia virus ]
• Official Symbol: B19R
• Synonyms: B19R; VACWR200; IFN-alpha/beta-receptor-like secreted glycoprotein
• Gene ID: 3707577
• Protein Refseq: YP_233082.1
• UniProt ID: P25213