Species : |
Xanthomonas manihotis |
Source : |
E. coli |
Tag : |
Non |
Description : |
α(1-6) Core Mannosidase cleaves unbranched non-reducing terminal mannose, α(1-6) linked to the β-linked core mannose of the conserved mannosylchitobiose core of N-linked oligosaccharides. The presence of fucose linked to the core N-acetylglucosamine does not affect cleavage.
The enzyme may inhibit other mannosidases, such as α(1-2,3,6) mannosidase, if a noncleavable α(1-6) mannose is present on the substrate. It should therefore always be added after digestion by other mannosidases. |
Form : |
Sterile-filtered solution |
EC : |
3.2.1.24 |
Specificity : |
Cleaves unbranched non-reducing terminal mannose, α(1-6) linked to the beta-linked core mannose of the conserved mannosylchitobiose core of N-linked oligosaccharides. The presence of fucose linked to the core N-acetylglucosamine has no effect on cleavage. The enzyme may inhibit other mannosidases if a noncleavable α(1-6) mannose is present on the substrate. It should therefore always be added subsequent to digestion by other mannosidases. |
Contents : |
Core Alpha Mannosidase in 50 mM sodium phosphate, 0.1 mM ZnCl (pH 7.5).
200 μL 5× Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0). |
Bio-activity : |
Specific Activity: > 1.5 U/mg
Activity: 1 U/mL |
Molecular Mass : |
~52 kDa |
Suggested usage : |
1. Add up to 1 nmol of oligosaccharide.
2. Add deionized water to 15 μL.
3. Add 4 μL 5× Reaction Buffer 5.0.
4. Add 1 μL of enzyme
5. Incubate ten minutes at 37 centigrade. |
Unit Definition : |
One unit of α(1-6) Mannosidase is defined as the amount of enzyme required to produce 2 μmoles of mannose in 1 minute at 37 centigrade, pH 5.0 from α(1-6) mannobiose. |
Applications : |
α(1-6) Core Mannosidase is useful for:
Determining mannose linkages
Rapidly removing the α(1-6) core mannose resistant to other mannosidases |
Stability : |
Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity. |
Storage : |
Store enzyme at 4 centigrade. Do not freeze. |
Storage Buffer : |
The enzyme is provided as a sterile solution in 50 mM sodium phosphate pH 7.5, 0.1 mM ZnCl2. |