Active Spike RBD (B.1.1.7) protein-coupled magnetic Beads

• Cat.No.: Spike-4132M

Specification

• Species: Sars-CoV-2
• Source: HEK293
• Protein Length: Arg 319 - Lys 537(N501Y)
• Appearance: Powder mixture
• Particle size: 2 μm
• Bio-activity: Immobilized 33 μg RBD protein /1mg beads can bind ACE2 with an EC50 of 1.337 μg/mL.
• Coupled amount of protein: ≈1194 pmol (33.8 μg) SARS-CoV-2 RBD protein/mg beads
• Capacity: >300 pmol (33 μg) Human ACE2 ligand/mg beads
• Formulation: PBS, pH7.4, with 10% Trehalose
• Reconstitution: 2 mL sterile deionized water (1 mg beads/mL)
• Background: The SARS-CoV-2 Spike RBD (B.1.1.7) Coupled Magnetic Beads is produced by coupling biotinylated SARS-CoV-2 spike RBD to streptavidin-conjugated magnetic beads. The spike RBD coupled to the beads contains the critical N501Y mutation identified in multiple circulating SARS-CoV-2 variants, e.g. the U.K. variant (known as B.1.1.7, 20B/501Y.V1 or VOC 202012/01), the South African variant (known as B.1.351 or 20C/501Y.V2) and the Brazilian variant (known as P.1). The pre-coupled beads are ready to use for capturing anti-SARS-CoV-2 antibody or ACE2 protein from your sample with high specificity.
• Application: This product is intended for immunocapture, biopanning and flow cytometry. This product is produced non-sterile.
• Storage: Upon receipt, please store the Beads at -20°C for 1 year in lyophilized state.