| Species : |
Chicken |
| Form : |
Liquid |
| Specificity : |
Tissues were washed exhaustively with PBS to remove blood and other debris. A lysate was prepared by homogenizing the tissue and washing the cells in cold PBS. Washed cells were incubated at 4 centigrade in modified RIPA buffer to lyse the cells. Protein integrity is ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 μM Aprotinin, 5 μM Bestatin, 1.5 μM E-64, 2 μM Leupeptin Hemisulfate and 1 μM Pepstatin A). The following phosphatase inhibitors were also added: 1 mM NaF and 1 mM Na3VO4. Cell debris was removed by centrifugation and membrane filtration. Protein concentration was determined by Lowry assay using a commercially available kit. The protein concentration was adjusted to 2 mg/mL and then an equal volume of 2× SDS-PAGE sample buffer was added. |
| Applications : |
SDS-PAGE |
| Cell Line : |
Primary tissue |
| Lysate Fractionation : |
Whole Cell Lysate |
| Lysate Stimulation : |
Not Stimulated |
| Culture Type : |
Primary Tissue |
| Induction : |
None (Control) |
| Concentration : |
1.0 mg/mL by modified Lowry assay |
| Buffer : |
1× SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2% SDS, 10% Glycerol and 0.005% bromophenol blue, pH 6.8) |
| Preservative : |
None |
| Stabilizer : |
10% (v/v) Glycerol |
| Shipping : |
Dry Ice |
| Storage : |
Store vial at -70 centigrade or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles. |
| Expiration : |
Expiration date is three months from date of receipt. |
