| Cat.No.: | Kit-0086 |
| Product Overview: | Ammonia Assay Kit is a quantitative fluorimetric determination of ammonia at FL360/450 nm. |
| Description: | AMMONIA (NH3) or its ion form ammonium (NH4+) is an important source of nitrogen for living systems and is ubiquitously present in the nature. Simple, direct and automation-ready procedures for measuring NH3 are very desirable. Ammonia Assay Kit is based on an improved o-phthalaldehyde method. This reagent reacts with ammonia/ ammonium and forms a fluorescent product. The fluorescence intensity (λex/em = 360/450nm) is proportional to the ammonia concentration in the sample. |
| Applications: | Ammonia/ammonium determination in biological (e.g. urine) and environmental samples. |
| Usage: | For research use only (RUO) |
| Storage: | Store kit at -20°C. Shelf life of 6 months after receipt. |
| Kit Components: | Assay Buffer 20 mLStandard (NH4Cl) 400 µLReagent A 1 mLReagent B 1 mL |
| Detection method: | Fluorimetric |
| Compatible Sample Types: | Environ-mental Sample, Urine |
| Features & Benefits: | Fast and sensitive. Linear detection range of 0.012 - 1 mM ammonia.Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. |
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