ACE1 Activity Assay Kit utilizes an active ACE1 to cleave a synthetic o-aminobenzoyl peptide (Abz-based peptide) substrate to release a fluorophore. The released Abz can be easily quantified using a fluorescence microplate reader. This assay kit is simple, rapid and can detect ACE activity as low as 10 mU in biological samples.
Detection of ACE1 activity in tissue/cell lysatesDetermination of enzymatic activity of purified ACE1
Store kit at -20°C, protected from light. Briefly centrifuge small vials at low speed prior to opening. Read entire protocol before performing the assay.• ACE1 Lysis Buffer and ACE1 Dilution Buffer: Ready to use. Store at -20ºC. Bring to room temperature before use.• ACE1 Lysis Buffer: Ready to use. Store at -20°C. Thaw before use.• ACE1 Positive Control: Store at -20°C. Avoid multiple freeze/thaw of the enzyme. Use within 6 months.• ACE1 Substrate: Ready to use. Store at -20°C. Thaw before use.• Abz Standard: Ready to use. Store at -20°C.