Angiotensin I Converting Enzyme Inhibitor Screening Kit
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|Product Overview :||ACE1 Inhibitor Screening Kit can be used to screen for potent inhibitors of ACE1 activity to regulate hypertension. It utilizes the ability of an active ACE1 to cleave a synthetic oaminobenzoyl peptide (Abz-based peptide substrate to release a free fluorophore). The released Abz can be easily quantified using a fluorescence microplate reader. In the presence of an ACE1 specific inhibitor, the enzyme loses its peptidase activity which results in decrease of fluorescence intensity. This assay kit is simple and can be used to identify and characterize ACE1 inhibitors in a highthroughput format.|
|Size :||100 assays|
|Applications :||Screening/studying/characterizing inhibitors of ACE1|
|Storage :||Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.• ACE1 Assay Buffer and ACE1 Dilution Buffer: Store at -20 °C or 4 °C. Bring to room temperature before use.• ACE1 Enzyme: Store at -20°C. Avoid multiple freeze/thaw of the enzyme. Use within 6 months.• ACE1 Substrate: Ready to use. Store at -20°C. Thaw before use.• ACE1 Inhibitor: Ready to use. Store at -20°C. Thaw before use.|
◆ Assay kits
|Kit-2112||Angiotensin I Converting Enzyme Activity Assay Kit||+Inquiry|