Bacterial Genomic DNA Isolation Kit

• Cat.No.: Kit-2105
• Product Overview: The bacterial genomic DNA isolation kit provides convenient and simple step-by-step method for isolating quality genomic DNA from gram-negative and gram-positive bacterial species. This kit utilizes enzymatic reactions to release bacterial DNA from the cell. DNA release from the cell is coupled with adsorption of DNA onto a silica spin-column in the presence of high salt concentration, eliminating the use of toxic organic compounds or solvents. DNA purified by this kit is suitable for various downstream molecular biology applications such as PCR, cloning, DNA hybridization, and Southern Blotting.

Specification

• Size: 100 isolations
• Applications: Useful for PCR, Cloning, DNA hybridization, Southern Blotting
• Storage: Store kit at -20°C and RT, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.Buffer A, B, and E: Ready to use. Store at room temperature.Buffer C: Add 28 mL of 100% Ethanol, molecular biology grade. Mix well and store at room temperature.Buffer D: Add 136 mL of 100% Ethanol, molecular biology grade. Mix well and store at room temperature.Enzyme Mix A, Enzyme Mix B, and RNAseA: Ready to use. Store at -20°C. Keep on ice at all times while in use.Spin Columns: Ready to use. Store at room temperature in dry conditions.