Bacterial Membrane Potential Kit


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Bacterial Membrane Potential Kit

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Cat.No. : Kit-1678
Product Overview : The reagents in the Bacterial Membrane Potential Kit have been tested at Molecular Probes on logarithmically growing cultures of the following bacterial species: Micrococcus luteus, Staphylococcus aureus, S. warnerii, Bacillus cereus, Klebsiella pneumoniae, Escherichia coli, and Salmonella choleraesuis. Many bacteria do not show a proportional response to partial membrane depolarization with DiOC2 (3). The response of each bacterial system should be investigated and optimized. Occasionally the DiOC2 (3) concentration and staining time must be adjusted for optimal detection of membrane potential. The experimental protocols below are provided as examples to guide researchers in the development of their own bacterial staining procedures. Some common buffer components, such as Tween 20, azide, and thimerosal, can alter membrane potential and should be avoided. Be sure to test buffer additives for their effect on membrane potential during optimization studies.
Size : 100 tests
Storage : Store the components of the BacLight Bacterial Membrane Potential Kit at room temperature before opening the vials. When stored properly, these kit components are stable for at least six months. Caution:DMSO stock solutions should be handled with particular care, as DMSO is known to facilitate the entry of organic molecules into tissues. Please dispose of the stains in compliance with all pertinent local regulations.
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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