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Beta Galactosidase (β-Gal) Activity Assay Kit (Fluorometric)

Cat.No.: Kit-1093
Description: Beta Galactosidase (β-Gal, EC: 3.2.1.23) is an enzyme which hydrolyzes the β-galactosides into monosacchanides. β-Gal is widely used as a reporter gene in the field of molecular biology. Senescence Associated β-Gal (SA-β-Gal) is an isoform of β-Gal which has the optimal activity at pH 6.0, and is mostly used as a biomarker for senescent cells. β-Gal is an essential enzyme in humans and its deficiency results in Morquio’s Syndrome, a severe birth defect. Beta Galactosidase Activity Assay kit provides a quick and easy way for monitoring Beta Galactosidase activity in a variety of samples. In this kit, Beta Galactosidase hydrolyses a non-fluorescent substrate to generate a strong fluorescent product. The assay is simple, sensitive, and high-throughput adaptable. Detection limit: < 0.1 mU.
Applications: Measurement of β-Galactosidase activity in various samples
Storage: -20°C
Shipping: Gel Pack
Size: 100 assays
Kit Components: β-Gal Assay Buffer; β-Gal Substrate (in DMSO); Fluorescein Standard (1 mM); β-Gal Positive Control
Detection method: Fluorescence (Ex/Em = 480/520 nm)
Features & Benefits: Quick and easy method to measure Beta Galactosidase activity.
Detection limit: < 0.1 mU

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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