"Bioluminescence" Related Products

Bioluminescence Cytotoxicity Assay Kit

Cat.No.: Kit-2255
Product Overview: Cell death or cytotoxicity is classically evaluated by the quantification of plasma membrane damage. Adenylate kinase (AK) is a ubiquitous protein present in all eukaryotic and prokaryotic cells and rapidly release into culture medium upon damage of the plasma membrane. The Bioluminescence Cytotoxicity Assay Kit is based on the measurement of AK in a simple one-step procedure involving two chemical reactions. The first reaction is the conversion of ADP to ATP by adenylate kinase that was released from the damaged cells. The second reaction utilizes luciferase to catalyze the formation of light from ATP and luciferin, and the light can be measured using a luminometer or beta counter. The assay is highly sensitive and can be fully automatic for high throughput.
Storage: -20°C
Shipping: Gel Pack
Size: 500 assays
Kit Components: AK Detection Reagent (Lyophilized); AK Assay Buffer
Target Species: Mammalian
Detection method: Luminescence
Features & Benefits: Simple one-step procedure; takes less than 30 minutes;
Fast and convenient;
The assay is highly sensitive and can be fully automatic for high throughput studies.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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