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Biotin-Capture Beads

Cat.No. : Avidin-11A
Product Overview : Biotin-Capture Beads contain modified Avidin that is immobilized on rigid, highly cross-linked beaded agarose possessing high chemical stability. Our modified Avidin provides a substantial improvement over native Avidin. The high affinity to biotin is maintained, while background problems are minimized due to chemical modification on the protein. The Biotin-Capture Beads has proven to be particularly useful in the isolation of antigens and nucleic acids by employing biotinylated antibodies, or nucleic acid probes respectively. Biotin-Capture Beads can be used in numerous additional affinity chromatography applications.
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Cat.no : Avidin-11A
Matrix : Highly cross-linked 4% spherical agarose
Functional group : Modified Avidin
Average particle size : 40-165 μm
Dynamic binding capacity : 2-4 mg biotinilated BSA per ml of beads
Recommended flow rate : 1-2 ml/min/cm2
pH working range : 2-11

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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What are the applications of Avidin in biotechnology? 09/19/2022

Avidin is widely used in biotechnology in the fields of biotin labeling, affinity purification, immunohistochemistry and so on. It uses its highly specific binding to biotin to achieve the detection and separation of specific molecules.

How does Avidin bind biotin? 05/14/2022

Avidin binds tightly to biotin through affinity sites in its four subunits, forming a very stable binding complex.

What happens to Avidin in the absence of biotin? 07/09/2020

In the case of biotin deficiency, Avidin may bind to biotin in the organism, resulting in the deficiency of biotin, which in turn affects the normal metabolism and function of the organism.

Is Avidin toxic? 04/02/2020

Itself is not toxic, but in some cases may cause some side effects if ingested in large quantities or used inappropriately. Therefore, attention should be paid to the dosage and method of use when using Avidin.

Is the binding of Avidin to biotin reversible? 03/26/2020

The binding of Avidin to biotin is very potent, so that under conventional conditions, the binding is irreversible. "However, binding can be made reversible by specific conditions, such as high temperature or acidity."

Are there multiple structural variants of Avidin? 01/31/2019

Yes, there are many structural variants of Avidin, including Streptavidin, NeutrAvidin, etc., which may differ in the characteristics, stability and affinity of binding biotin.

Customer Reviews (3)

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Reviews
07/22/2022

    The purity is very high and there are no impurities.

    08/08/2020

      The structure is very stable and not prone to mutation.

      09/15/2019

        Due to biological activity is very strong and can be used in various biological experiments.

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