"Calcium" Related Products

Calcium Quantification Assay kit (Fluorometric)

Cat.No.: Kit-0141
Product Overview: The kit provides a simple method for detecting calcium in physiology solutions by using our proprietary red fluorescence probe. The fluorescence signal can be easily read by a fluorescence microplate reader at Ex/Em540/590 nm. The kit can be performed in a convenient 96-well or 384-well microtiter-plate format and easily adapted to automation without a separation step. The assay can be completed within 30 minutes. With ab112115, we have detected as little as 0.03 mM calcium. The kit has a broad dynamic range (30 μM to 1 mM).
Size: 200 tests
Description: This product is intended to be used in vitro with solution-based samples such as cell extracts. If you would like to detect calcium in vivo using live cells, please check our Fluo-8, Rhod-4 or Fura-2 based assays.
Applications: Functional Studies more details
Storage: Store at -20°C.
Kit Components: Components: 200 tests; 300 mM Calcium Standard: 1 x 0.5ml; Assay Buffer: 1 x 10ml; Rhod Red Indicator: 2 vials
Compatible Sample Types: Cell culture extracts
Assay time: 0h 30m
Sensitivity: 0.03 mM

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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