Casein, TAMRA-conjugated
Cat.No. : | DYE-137 |
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- Gene Information
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Description : | In the intact substrate, casein is heavily labeled with TAMRA, resulting in almost total quenching of the conjugate"s fluorescence. Compared to the fluoresceinated casein, TAMRA-labeled casein demonstrates pH-insensitive fluorescence. It is readily used for continuous fluorometric measurement of protease activity. Protease-catalyzed hydrolysis relieves this quenching conjugate, yielding brightly red fluorescent dye-labeled peptides. The increase in fluorescence intensity is directly proportional to protease activity. The lightly TAMRA-labeled casein may be useful for a continuous assay if monitored by fluorescence polarization. |
Ex(nm) : | 545 |
Em(nm) : | 576 |
Solvent : | Water |
Storage : | Store at -20°C in the dark. Dessicated. |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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