"Caspase" Related Products

Caspase-12 Fluorometric Assay Kit

Cat.No.: Kit-0158
Product Overview: Fluorometric assay kit for caspase-12 activity
Size: 1 kit/100 assays
Description: Caspase family of proteases are the central mediators of apoptosis in mammalian cells. The Caspase-12 Fluorometric Assay Kit provides a simple and convenient means for assaying the activity of caspases that recognize the sequence ATAD. The assay is based on detection of cleavage of substrate ATAD-AFC (AFC: 7-amino-4-trifluoromethyl coumarin). ATAD-AFC emits blue light (max = 400 nm); upon cleavage of the substrate by caspase-12 or related caspases, free AFC emits a yellow-green fluorescence (max = 505 nm), which can be quantified using a fluorometer or a fluorecence microtiter plate reader.
Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
Target Species: Mammal
Usage: For research use only. Not intended for any diagnostic or therapeutic purpose.
Storage: Store kit at -20°C (Store Cell Lysis Buffer & 2X Reaction Buffer at 4°C after opening). All reagents are Stable for 6 months from date of receipt under proper storage conditions.
Kit Components: Cell Lysis Buffer: 100ml;2X Reaction Buffer: 4*2ml; ATAD-AFC (1mM): 0.5ml; DTT (1M): 0.4ml
Detection method: Fluorecence microtiter plate reader (400-nm excitation filter and 505-nm emission filter) and fluorescence microscopy
Compatible Sample Types: Cell and tissue lysates
Features & Benefits: 1. Simple one-step procedure; takes only 1-2 hours.2. Fast and convenient.3. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-12 activity.

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.