Caspase-2 Colorimetric Assay Kit

• Cat.No.: Kit-1119

Specification

• Tag: Non
• Description: Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2 Colorimetric Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VDVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm.
• Applications: Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis.
• Storage: -20°C
• Shipping: Gel Pack
• Kit Components: Cell Lysis Buffer; 2X Reaction Buffer; VDVAD-pNA (4 mM); DTT (1 M); Dilution Buffer
• Target Species: Mammalian
• Detection method: Absorbance (400 or 405 nm)
• Features & Benefits: Simple one-step procedure; takes 1-2 hours;
  Fast and convenient;
  Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-2 activity.