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See All Caspase-2 Related Kits | Cat.No.: | Kit-1119 |
| Description: | Activation of ICE-family proteases/caspases initiates apoptosis in mammalian cells. The Caspase-2 Colorimetric Assay Kits provide a simple and convenient means for assaying the activity of caspases that recognize the sequence VDVAD. The assay is based on spectrophotometric detection of the chromophore p-nitroanilide (pNA) after cleavage from the labeled substrate VDVAD-pNA. The pNA light emission can be quantified using a spectrophotometer or a microtiter plate reader at 400- or 405 nm. |
| Applications: | Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. |
| Storage: | -20°C |
| Shipping: | Gel Pack |
| Kit Components: | Cell Lysis Buffer; 2X Reaction Buffer; VDVAD-pNA (4 mM); DTT (1 M); Dilution Buffer |
| Target Species: | Mammalian |
| Detection method: | Absorbance (400 or 405 nm) |
| Features & Benefits: | Simple one-step procedure; takes 1-2 hours; Fast and convenient; Comparison of the absorbance of pNA from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-2 activity. |
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