| Cat.No.: | Kit-2304 |
| Product Overview: | Caspases play a key role in apoptosis. The CaspSELECT; Caspase-7 Immunoassay Kit provides an effective immunosorbent enzyme assay for the specific, quantitative detection of caspase-7 activity in microtiter plates. The assay utilizes caspase-7 polyclonal antibody to capture activated caspase-7 from cell lysates. Substrate DEVD-AFC is then added that is cleaved proportionally to the amount of activated caspase-7 in the cell lysate. The cleavage generates free AFC which is then analyzed fluorometrically (Ex./Em. = 400/505 nm) using a fluorescence plate reader. The assay ensures absolute specific detection of caspase-7. Other known caspases and non-specific proteases are not detected. |
| Applications: | Effective immunosorbent enzyme assay for specific, quantitative detection of caspase-7 activity in microtiter plates. |
| Storage: | -20°C |
| Shipping: | Gel Pack |
| Size: | 100 assays |
| Kit Components: | Cell Lysis Buffer; Coating Buffer; Anti-Caspase-7 Antibody (20X); Blocking Buffer; Incubation Buffer; DTT (1 M); DEVD-AFC Substrate (1 mM); Positive Control (rh-Caspase-7); Microtiter Plate; Adhesive Plate Cover |
| Target Species: | Mammalian |
| Detection method: | Fluorescence(Ex/Em 370-425/ 490-525nm) |
| Features & Benefits: | Simple one-step procedure; takes only 2-4 hours; Fast and convenient |
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