Cathepsin E Activity Assay Kit (Fluorometric)

• Cat.No.: Kit-1055

Specification

• Tag: Non
• Description: Cathepsin E (CTSE, EC: 3.4.23.34) is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that is found in highest concentration on the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. Cathepsin E Activity Assay Kit utilizes the ability of an active Cathepsin E to cleave a synthetic MCA-based peptide substrate to release free MCA, which can be easily quantified using a fluorometer or fluorescence microplate reader. This assay kit is simple, rapid and can detect Cathepsin E activity as low as 1 mU in biological samples.
• Applications: Detection of Cathepsin E activity in tissue and cell lysatesDetermine activity of purified Cathepsin E
• Storage: -20°C
• Shipping: Gel Pack
• Size: 100 assays
• Kit Components: CTSE Assay Buffer; CTSE Lysis Buffer; CTSE Substrate; Human Cathepsin E (Positive Control); MCA Standard (1 mM)
• Detection method: Fluorescence (Ex/Em = 320/420 nm)
• Features & Benefits: Rapid & convenient;
  Detects Cathepsin E activity as low as 1 mU in biological samples