Cathepsin E Fluorometric Activity Assay Kit

• Cat.No.: Kit-2145
• Product Overview: Cathepsin E Activity Assay Kit utilizes the ability of an active Cathepsin E to cleave a synthetic MCA-based peptide substrate to release free MCA, which can be easily quantified using a fluorometer or fluorescence microplate reader. This assay kit is simple, rapid and can detect Cathepsin E activity as low as 1 mU in biological samples.

Specification

• Size: 100 assays
• Applications: Detection of Cathepsin E activity in tissue and cell lysatesDetermine activity of purified Cathepsin E
• Storage: Store kit at -20°C, protected from light. Briefly centrifuge small vials at low speed prior to opening. Read entire protocol before performing the assay.CTSE Assay Buffer and Lysis Buffer: Bring to room temperature before use. Store at -20°C.Human Cathepsin E: Reconstitute with 20 µl of de-ionized water. Aliquot and store at -20°C. Avoid repeated freeze/thaw. Use within two months.