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Cathepsin E Fluorometric Activity Assay Kit

Cat.No.: Kit-2145
Product Overview: Cathepsin E Activity Assay Kit utilizes the ability of an active Cathepsin E to cleave a synthetic MCA-based peptide substrate to release free MCA, which can be easily quantified using a fluorometer or fluorescence microplate reader. This assay kit is simple, rapid and can detect Cathepsin E activity as low as 1 mU in biological samples.
Size: 100 assays
Description: Cathepsin E (CTSE, EC: 3.4.23.34) is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that is found in highest concentration on the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers.
Applications: Detection of Cathepsin E activity in tissue and cell lysatesDetermine activity of purified Cathepsin E
Storage: Store kit at -20°C, protected from light. Briefly centrifuge small vials at low speed prior to opening. Read entire protocol before performing the assay.CTSE Assay Buffer and Lysis Buffer: Bring to room temperature before use. Store at -20°C.Human Cathepsin E: Reconstitute with 20 µl of de-ionized water. Aliquot and store at -20°C. Avoid repeated freeze/thaw. Use within two months.
Kit Components: CTSE Assay Buffer: 25 mlCTSE Lysis Buffer: 25 mlCTSE Substrate: 0.2 mlHuman Cathepsin E (Positive Control): 1 vialMCA Standard (1 mM): 15 μl
Detection method: Fluorescence (Ex/Em = 320/420 nm)
Compatible Sample Types: • Cell and tissue lysates• Purified Cathepsin E
Features & Benefits: • Rapid & convenient• Detects Cathepsin E activity as low as 1 mU in biological samples

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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