| Tag : |
Non |
| Size : |
100 assays |
| Description : |
Cathepsin E (CTSE, EC: 3.4.23.34) is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that is found in highest concentration on the surface of epithelial mucus-producing cells of the stomach. It is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. |
| Applications : |
Detection of Cathepsin E activity in tissue and cell lysatesDetermine activity of purified Cathepsin E |
| Storage : |
Store kit at -20°C, protected from light. Briefly centrifuge small vials at low speed prior to opening. Read entire protocol before performing the assay.CTSE Assay Buffer and Lysis Buffer: Bring to room temperature before use. Store at -20°C.Human Cathepsin E: Reconstitute with 20 µl of de-ionized water. Aliquot and store at -20°C. Avoid repeated freeze/thaw. Use within two months. |
| Kit Components : |
CTSE Assay Buffer: 25 mlCTSE Lysis Buffer: 25 mlCTSE Substrate: 0.2 mlHuman Cathepsin E (Positive Control): 1 vialMCA Standard (1 mM): 15 μl |
| Detection method : |
Fluorescence (Ex/Em = 320/420 nm) |
| Compatible Sample Types : |
• Cell and tissue lysates• Purified Cathepsin E |
| Features & Benefits : |
• Rapid & convenient• Detects Cathepsin E activity as low as 1 mU in biological samples |
