"Cell" Related Products

Cell Transformation Assay Kit (Colorimetric)

Cat.No.: Kit-1051
Description: The transformed cells can proliferate without attaching to surface. Anchorage independent cell growth is the hallmark of cell transformation. The Soft-Agar Assay is a traditional method for screening cell transformation in vitro. However, this method is lengthy (3-4 weeks incubation), laborious (counting colonies) and inconsistent (due to subjective counting). Cell Transformation Assay is faster, stable, and more sensitive than the traditional soft-agar assay. The kit is based on the conversion of the tetrazolium salt (WST-8) to formazan by cellular mitochondrial dehydrogenases. The generated signal is directly proportional to the number of living cells. This one-step method is non-radioactive and simple (just add-and-read, does not require harvesting cells, and solubilization steps). The assay is high-throughput adaptable and has wide linear range from 10000-400000 cells. The entire Cell Transformation Assay can be finished within 7-8 days.
Applications: Measure cell transformation in response to stimuli;
Screen and characterize compounds that influence cell transformation
Storage: -20°C
Shipping: Gel Pack
Size: 100 assays
Kit Components: Agarose Powder DMEM Solution (10X); Staining Solution; WST Reagent; Electro Coupling Solution (ECS)
Target Species: Mammalian cells
Detection method: Colorimetric (OD 450 nm)
Features & Benefits: Highly sensitive fluorometric method to measure cell transformation in response to a variety of biochemical stimuli;
Simple & High throughput-adaptable;
Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect cell transformation

Online Inquiry

Note: There will be extra charge for optional service!
Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Price does not include shipping and packaging costs.Request quote for final price.

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.