"Cell" Related Products

Cell Transformation Assay Kit (Fluorometric)

Cat.No.: Kit-1052
Description: Transformed cells can proliferate without attaching to surface Anchorage-independent cell growth is the hallmark of cell transformation. The Soft-Agar Assay is a traditional method for screening cell transformation in vitro. However, this method is lengthy (3-4 weeks incubation), laborious (counting colonies) and inconsistent (due to subjective counting). Cell Transformation Assay is faster, stable, and more sensitive than the traditional soft-agar assay. The kit uses a quantitative dye that binds to nucleic acid and generates green fluorescence. This one-step method is non-radioactive and simple (just add-and-read, and does not require tedious labor such as counting colonies). The assay is high-throughput adaptable and has wide linear range from 50-60,000 cells. The entire assay can be finished within 7-8 days.
Applications: Measure cell transformation in response to stimuli;
Screen and characterize compounds that influence cell transformation
Storage: -20°C
Shipping: Gel Pack
Size: 100 assays
Kit Components: Agarose Powder; DMEM Solution (10X); Staining Solution; Agarose Solubilization Solution; Quantitative Dye (200X)
Target Species: Mammalian cells
Detection method: Fluorescence (Ex/Em = 480/530 nm)
Features & Benefits: Highly sensitive fluorometric method to measure cell transformation in response to a variety of biochemical stimuli;
Simple & High throughput-adaptable;
Reproducible, Quantitative tool for screening, studying, and characterizing compounds that affect cell transformation

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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