Cellular Calcineurin Phosphatase Activity Assay Kit (Colorimetric) is a complete colorimetric assay kit designed for measuring cellular calcineurin (CaN, PP2B) phosphatase activity. It employs a convenient 96-well microtiter-plate format with all reagents necessary for measuring calcineurin (PP2B) phosphatase activity in tissue/cellular extracts. The detection of free-phosphate released is based on the classic Malachite green assay and offers the following advantages: Non-radioactive; convenient 1-step detection; excellent sensitivity. This kit includes human recombinant calcineurin as a positive control. The RII phosphopeptide substrate (Calcineurin Substrate), supplied with this kit, is the most efficient and outstanding peptide substrate known for calcineurin.