|Product Overview:||CITRATE is an intermediate in the citric acid cycle and is involved in fatty acid synthesis. The Citrate Assay Kit provides a simple, and automation-ready procedure for measuring citrate concentration. Citrate is converted into pyruvate which is then oxidized with the conversion of the dye into a colored and fluorescent form. The color intensity at 570 nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the citrate concentration in the sample.|
|Detection method:||OD570nm, or FL530/585nm|
|Compatible Sample Types:||Serum, plasma, agriculture etc|
|Features & Benefits:||Fast and sensitive. Linear detection range: 4 to 400 μM citrate for colorimetric assays and 0.5 to 40 μM for fluorimetric assays.
Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
|Assay time:||20 min|
|Sensitivity:||OD, FL: 4, 0.5 μM.|