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Citrate Synthase Activity Colorimetric Assay Kit

Cat.No.: Kit-2281
Product Overview: Citrate Synthase (EC 2.3.3.1) is a key enzyme that is present in all living organisms. It catalyzes the conversion of acetyl-CoA and oxaloacetate into citrate and serves as a marker for intact mitochondria. A recent study showed that increased activity of mitochondrial Citrate Synthase is directly associated with metabolic and endocrine abnormalities such as obesity. In Citrate Synthase Activity Assay Kit, Citrate Synthase reacts with substrate mix to form an intermediate, which subsequently reacts with developer to generate the colored product. The rate of color development is proportional to the enzyme activity. The assay is simple, rapid and can detect Citrate Synthase activity less than 1 mU in a variety of samples.
Applications: Measurement of citrate synthase activity in various tissues/cellsAnalysis of intact mitochondria.
Storage: -20°C
Shipping: Gel Pack
Size: 100 assays
Kit Components: CS Assay Buffer; CS Substrate Mix (Lyophilized); CS Developer (Lyophilized); GSH Standard (reduced) (Lyophilized); CS Positive Control (Lyophilized)
Detection method: Absorbance (412 nm)
Features & Benefits: Simple, rapid & convenient;
The assay can detect Citrate Synthase Activity less than 1 mU in a variety of samples

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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