Citric Acid (OA) Content Assay Kit

• Cat.No.: KITZ-074
• Product Overview: Oxalic acid is a dicarboxylic acid commonly found in plant tissues. As an antagonist of mineral elements, consuming vegetables with high oxalic acid content over a long period can easily lead to diseases such as arthritis, hypocalcemia, and kidney stones. Oxalic acid can serve as a complexing agent, bleaching agent, precipitating agent, and reducing agent, and it is widely used in industries such as pharmaceuticals, dyeing, and plastics. Fe3+ can react with sulfosalicylic acid under acidic conditions to form a purple complex, which has a characteristic absorption peak at 510 nm. Oxalic acid and oxalate can lighten the color of this purple complex, and the absorbance value of the Fe3+ and sulfosalicylic acid complex decreases with the increase of oxalic acid. By detecting changes in absorbance, the oxalic acid content can be determined.

Specification

• Size: 48 Samples
• Storage: 4°C
• Shipping: Ice pack
• Kit Components: Extraction Liquid: Liquid, 60mL × 1 bottle, store at 4°C.
  
  Reagent One: Powder, mg × 1 vial, store at 4°C. Before use, shake to bring the powder to the bottom, then add 1.5mL of distilled water to dissolve and reserve.
  
  Reagent Two: Liquid, 20mL × 1 bottle, store at 4°C.
  
  Reagent Three: Liquid, 1.5mL × 1 vial, store at 4°C.
  
  Standard: Powder, mg × 1 vial, store at 4°C. This reagent is used if recalibration of the standard curve is necessary.
• Materials Required but Not Supplied: Microplate reader, 96-well plate, benchtop centrifuge, adjustable pipette, water bath/metal bath, mortar, ice, distilled water.