"Complex" Related Products

Complex IV Human Enzyme Activity Microplate Assay Kit

Cat.No.: Kit-0243
Product Overview: The Complex IV Human Enzyme Activity Microplate Assay Kit is used to determine the activity of cytochrome c oxidase in a human sample with greater speed and simplicity. The COX enzyme is immunocaptured within the wells of the microplate and activity is determined colorimetrically by following the oxidation of reduced cytochrome c by the absorbance change at 550 nm. Included in this kit for performance of the activity assay are buffer, detergent, substrate, and 96-well microplate with monoclonal antibody pre-bound to the wells of the plate, allowing for a stream-lined assay.
Size: 1 x 96 well plate
Applications: Functional Studies
Target Species: Reacts with: Cow, Human, PigDoes not react withMouse, Rat
Storage: Reagent C should be aliquoted and stored at -20°C or preferably at-80°C. Tube 1, Detergent and the covered microplate should bestored at 4°C-DO NOT FREEZE.
Kit Components: Components 96 tests96-well microplate (12 strips) 1 unitBuffer 1 x 10mlDetergent 1 x 1mlReagent C (Cytochrome c) 1 x 1ml
Detection method: Colorimetric
Compatible Sample Types: Cell culture extracts, Tissue

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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