s D-2-Hydroxyglutarate Assay Kit provides a convenient method to detect D2HG in biological samples. In this assay, D-2-Hydroxyglutarate is oxidized to α-Ketoglutarate in the presence of D2HG Enzyme and Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at 450 nm. This absorbance is proportional to the amount of D2HG present in the samples. This assay kit is fast, sensitive, easy to use and high-through adaptable,It can measure D-2-Hydroxyglutarate levels less than 10 µM in various samples.
Measurement of D2HG level in various cell/tissues/biological fluids.
Store kit at -20°C, protected from light. Bring the D2HG Assay Buffer to room temperature before use. Briefly centrifuge all small vials prior to opening. Read the entire protocol before the assay.