"D2HG" Related Products

        D-2-Hydroxyglutarate Colorimetric Assay Kit

        Cat.No. : Kit-2120
        Product Overview : s D-2-Hydroxyglutarate Assay Kit provides a convenient method to detect D2HG in biological samples. In this assay, D-2-Hydroxyglutarate is oxidized to α-Ketoglutarate in the presence of D2HG Enzyme and Substrate Mix. The intermediate reduces the probe to a colored product with strong absorbance at 450 nm. This absorbance is proportional to the amount of D2HG present in the samples. This assay kit is fast, sensitive, easy to use and high-through adaptable,It can measure D-2-Hydroxyglutarate levels less than 10 µM in various samples.
        Size : 100 assays
        Description : In eukaryotic cells, Isocitrate Dehydrogenase (IDH1, IDH2 and IDH3) catalyzes the interconversion of Isocitrate and α-Ketoglutarate. In human cancers, an IDH mutation causes a gain-of-function, which reduces its affinity for isocitrate and facilitates the conversion of α- ketoglutarate to D-2-Hydroxyglutarate in the presence of NADP. D-2-Hydroxyglutarate (D2HG) is present at low level in normal cells and tissues, but is significantly elevated in metabolic diseases and various cancers. Therefore, detection of elevated D2HG is important for early diagnosis, prognosis and the development of therapeutic strategies against these maladies.
        Applications : Measurement of D2HG level in various cell/tissues/biological fluids.
        Target Species : Mammalian
        Storage : Store kit at -20°C, protected from light. Bring the D2HG Assay Buffer to room temperature before use. Briefly centrifuge all small vials prior to opening. Read the entire protocol before the assay.
        Kit Components : D2HG Assay Buffer: 20 mlD2HG Enzyme: 1 vialD2HG Substrate Mix: 1 vialD2HG Standard: 1 vial
        Detection method : Absorbance (OD = 450 nm)
        Compatible Sample Types : • Adherent or suspension Cells: e.g. 3T3, HepG2, Jurkat cells.• Tissues: e.g. Rat Liver, Rat Kidney, etc.• Biological Samples: Urine.
        Features & Benefits : • Simple, fast, HTP adaptable and convenient assay• Can measure D2HG levels as low as 10 µM in various samples.

        Online Inquiry

        Note: There will be extra charge for optional service!

        Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

        Optional requirements on this protein    +Expand

        Price Inquiry

        Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.