|Product Overview:||D-Lactate is the result of anaerobic glycolysis by microorganisms in the gastrointestinal system, and the product of detoxification of methylglyoxal by the glyoxalase system. The presence of abnormal levels of D-Lactate has been linked to a series of pathological conditions, such as diabetes, and appendicitis. D-Lactate Assay kit offers simplicity, sensitivity, and can be adapted to high-throughput research. The assay enzymatically oxidizes D-Lactate generating a fluorescent signal (Ex/Em = 535/587 nm). The signal is directly proportional to the amount of D-Lactate. The assay kit can detect samples containing as low as 0.1 μM D-Lactate.|
|Applications:||Measurement of D-Lactate in various tissues/cells/biological fluids. Analysis of D-Lactate in pathological conditions. Mechanistic study of the glyoxalase system.|
|Kit Components:||D-Lactate Assy Buffer; (in DMSO); D-Lactate Enzyme Mix (Lyophilized); D-Lactate Substrate Mix (Lyophilized); D-Lactate Standard (100 mM)|
|Detection method:||Fluorescence (Ex/Em= 535/587 nm)|
|Features & Benefits:||Simple, highly sensitive, and high-throughput adaptable.
The assay kit can detect samples containing as low as 0.1 μM D-Lactate.