"SurA" Related Products

Recombinant E.coli SurA, His-tagged

Cat. No. : SurA-2759E
Product Overview : Recombinant E.coli SurA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.
Description : SurA is a periplasmic peptidyl-prolyl isomerase (PPIase) and chaperone of Escherichia coli and other Gram-negative bacteria. This protein is a major factor in the biogenesis of beta-barrel outer membrane proteins (OMPs) and plays an integral role in cell envelope homeostasis and cell envelope functions. It is essential for the survival of E.coli in stationary phase and required for pilus biogenesis.
Source : E.coli.
Purity : > 95% by SDS – PAGE.
Sequences : MGSSHHHHHH SSGLVPRGSH MAPQVVDKVA AVVNNGVVLE SDVDGLMQSV KLNAAQARQQ LPDDATLRHQ IMERLIMDQI ILQMGQKMGV KISDEQLDQA IANIAKQNNM TLDQMRSRLA YDGLNYNTYR NQIRKEMIIS EVRNNEVRRR ITILPQEVES LAQQVGNQND ASTELNLSHI LIPLPENPTS DQVNEAESQA RAIVDQARNG ADFGKLAIAH SADQQALNGG QMGWGRIQEL PGIFAQALST AKKGDIVGPI RSGVGFHILK VNDLRGESKN ISVTEVHARH ILLKPSPIMT DEQARVKLEQ IAADIKSGKT TFAAAAKEFS QDPGSANQGG DLGWATPDIF DPAFRDALTR LNKGQMSAPV HSSFGWHLIE LLDTRNVDKT DAAQKDRAYR MLMNRKFSEE AASWMQEQRA SAYVKILSN.
Molecular Weight : 47.3 kDa(429aa), confirmed by MALDI-TOF.
Form : Liquid. In 20 mM Tris-HCl Buffer (pH 8.0) containing 10% Glycerol.
Concentration : 1 mg/ml (determined by Bradford assay).
Biological activity : Specific activity is > 450 nmoles/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAFP-pNA per minute at 25oC in Tris-Hcl pH8.0 using chymotrypsin.
Activity Assay : 1. Prepare 170ul assay buffer into a suitable container and pre-chill on ice before use: The final concentrations are 200 mM Tris-Hcl, pH 8.0, and 20nM chymotrypsin.2. Add 10ul of recombinant surA protein with 1ug in assay buffer.3. Mix by inversion and equilibrate to 1oC and monitor the A405nm until the value is constant using a spectrophotometer.4. Add 20ul pre-chilled 5mM suc-AAFP-pNA. (Substrate was dissolved in TFE that contained 460mM LiCl to a concentration of 3 mM)5. Record the increase in A405 nm for 30 minutes at 25oC.
Storage : Can be stored at +4oC short term (1-2 weeks). For long term storage, aliquot and store at -20oC or -70oC. Avoid repeated freezing and thawing cycles.
Gene Name : surA peptidyl-prolyl cis-trans isomerase SurA [ Escherichia coli UMN026 ]
Gene ID : 7257404
Synonyms : surA; peptidyl-prolyl cis-trans isomerase SurA; peptidyl-prolyl cis-trans isomerase SurA
Protein Refseq : YP_002410833

Online Inquiry

Note: There will be extra charge for optional service!

Please input "biomart" as verification code. Please review Creative BioMart's privacy policy for more information

Optional requirements on this protein    +Expand

Price Inquiry

Welcome! For price inquiries, please feel free to contact us through the form below. We will get back to you as soon as possible.