||Recombinant human PDXP protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques.
||Pyridoxal phosphate phosphatase, also known as PDXP is the enzyme belongs to the family of hydrolases, specifically those acting on phosphoric monoester bonds. PDXP catalyzes the dephosphorylation of pyridoxal 5"-phosphate and exhibits a high level of expression various parts of the central nervous system, especially the brain. PDXP activity is catalyzed by haloacid dehalogenase (HAD), and it is the cofactor of both aromatic L-amino acid decarboxylase and glutamate decarboxylase.
||> 95% by SDS – PAGE.
||MGSSHHHHHH SSGLVPRGSH MARCERLRGA ALRDVLGRAQ GVLFDCDGVL WNGERAVPGA PELLERLARA GKAALFVSNN SRRARPELAL RFARLGFGGL RAEQLFSSAL CAARLLRQRL PGPPDAPGAV FVLGGEGLRA ELRAAGLRLA GDPSAGDGAA PRVRAVLVGY DEHFSFAKLR EACAHLRDPE CLLVATDRDP WHPLSDGSRT PGTGSLAAAV ETASGRQALV VGKPSPYMFE CITENFSIDP ARTLMVGDRL ETDILFGHRC GMTTVLTLTG VSRLEEAQAY LAAGQHDLVP HYYVESIADL TEGLED.
||33.8 kDa (316aa) confirmed by MALDI-TOF.
||Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT,20% glycerol, 0.1M NaCl.
||1 mg/ml (determined by Bradford assay).
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.