Recombinant E. coli Chaperone, Periplasmic, His-tagged

• Cat.No.: fimC-3402E
• Product Overview: Recombinant E. coli FIMC protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Description: The 23 kDa two-domain periplasmic chaperone FIMC from Escherichia coli is required for the assembly of type-1 pili, which are filamentous, highly oligomeric protein complexes anchored to the outer bacterial membrane that mediate adhesion of pathogenic E. coli strains to host cell surfaces and persist in macrophages.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT.
• Molecular Weight: 25 kDa (226aa) confirmed by MALDI-TOF.
• Purity: > 95 % by SDS - PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH MGVALGATRV IYPAGQKQEQ LAVTNNDENS TYLI QSWVEN ADGVKDGRFI VTPPLFAMKG KKENTLRILD ATNNQLPQDR ESLF WM NVKA IPSMDKSKLT ENTLQLAIIS RIKLYYRPAK LALPPDQAAE KLRFRRSANS LTLI NPTPYY LTVTELNAGT RVLENALVPP MGESTVKLPS DAGSNITYRT INDYGALTPK MTGVME.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: fimC chaperone, periplasmic [ Escherichia coli str. K-12 substr. MG1655 ]
• Synonyms: chaperone, periplasmic; ECK4307; JW4279; periplasmic chaperone, required for type 1 fimbriae; fimC
• Gene ID: 948843
• Protein Refseq: NP_418736