Recombinant Human Activating Transcription Factor 1, His-tagged ATF1-1374H

Recombinant Human Activating Transcription Factor 1, His-tagged

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Recombinant Human Activating Transcription Factor 1, His-tagged

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Cat. No. : ATF1-1374H
Product Overview : Recombinanthuman ATF1protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques.
Description : ATF1 is a cyclic-AMPdependent transcription factor. ATF1 is expressed in a wide variety of celltypes and is capable of dimerizing with CREB. MSK1 and MSK2 protein kinasesare required for the stress-induced phosphorylation of transcription factorsCREB and ATF1 in primary embryonic fibroblasts. Epidermal growth factorinduction of c-jun expression requires ATF1 and MEF2 sites in the c-junpromoter.
Form : Liquid. In 20 mMTris-HCl buffer (pH 8.0) containing 5 mM DTT, 50% glycerol, 0.2M NaCl, 2 mM EDTA.
Molecular Weight : 31.8 kDa(295 aa), confirmed by MALDI-TOF.
Purity : > 90%by SDS-PAGE
Concentration : 0.25mg/ml(determined by Bradford assay)
Sequences of aminoacids : MGSSHHHHHHSSGLVPRGSH MGSHMEDSHK STTSETAPQP GSAVQGAHIS HIAQQVSSLS ESEESQDSSDSIGSSQKAHG ILARRPSYRK ILKDLSSEDT RGRKGDGENS GVSAAVTSMSVPTPIYQTSS GQYIAIAPNG ALQLASPGTD GVQGLQTLTM TNSGSTQQGT TILQYAQTSD GQQILVPSNQVVVQTASGDM QTYQIRTTPS ATSLPQTVVM TSPVTLTSQT TKTDDPQLKR EIRLMKNREA ARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLY SNKSV
Storage : Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Pathways : ActivatedTLR4 signalling; CREB phosphorylation; EGFR1 Signaling Pathway; ErbB1downstream signaling; HTLV-I infection; IL-3 Signaling Pathway; ImmuneSystem; Innate Immunity Signaling; MAP kinase activation in TLR cascade;MyD88 cascade initiated on plasma membrane; Myometrial Relaxation andContraction Pathways; NGF signalling via TRKA from the plasma membrane;Serotonin Receptor 4/6/7 and NR3C Signaling; Signalling by NGF; TRAF6Mediated Induction of proinflammatory cytokines; Toll Like Receptor 10(TLR10) Cascade; Toll Like Receptor 2 Cascade
PDB rendering based on 1dh3. :

Gene Name : ATF1 activating transcriptionfactor 1 [ Homo sapiens ]
Official Symbol : ATF1
Synonyms : ATF1; activatingtranscription factor 1; TREB36; EWS-ATF1;FUS/ATF-1; cyclic AMP-dependent transcription factor ATF-1; cAMP-dependenttranscription factor ATF-1
Gene ID : 466
mRNA Refseq : NM_005171
Protein Refseq : NP_005162
MIM : 123803
UniProt ID : P18846
Chromosome Location : 12q13
Function : proteincomplex binding ; protein heterodimerization activity ; sequence-specific DNAbinding ; sequence-specific DNA binding transcription factor activity ;sequence-specific distal enhancer binding RNA polymerase II transcriptionfactor activity

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