| Species : |
Human |
| Source : |
E.coli |
| Tag : |
His |
| Description : |
ATF1 is a cyclic-AMPdependent transcription factor. ATF1 is expressed in a wide variety of celltypes and is capable of dimerizing with CREB. MSK1 and MSK2 protein kinasesare required for the stress-induced phosphorylation of transcription factorsCREB and ATF1 in primary embryonic fibroblasts. Epidermal growth factorinduction of c-jun expression requires ATF1 and MEF2 sites in the c-junpromoter. |
| Form : |
Liquid. In 20 mMTris-HCl buffer (pH 8.0) containing 5 mM DTT, 50% glycerol, 0.2M NaCl, 2 mM EDTA. |
| Molecular Weight : |
31.8 kDa(295 aa), confirmed by MALDI-TOF. |
| Purity : |
> 90%by SDS-PAGE |
| Concentration : |
0.25mg/ml(determined by Bradford assay) |
| Sequences of aminoacids : |
MGSSHHHHHHSSGLVPRGSH MGSHMEDSHK STTSETAPQP GSAVQGAHIS HIAQQVSSLS ESEESQDSSDSIGSSQKAHG ILARRPSYRK ILKDLSSEDT RGRKGDGENS GVSAAVTSMSVPTPIYQTSS GQYIAIAPNG ALQLASPGTD GVQGLQTLTM TNSGSTQQGT TILQYAQTSD GQQILVPSNQVVVQTASGDM QTYQIRTTPS ATSLPQTVVM TSPVTLTSQT TKTDDPQLKR EIRLMKNREA ARECRRKKKEYVKCLENRVAVLENQNKTLIEELKTLKDLY SNKSV |
| Storage : |
Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
| Pathways : |
ActivatedTLR4 signalling; CREB phosphorylation; EGFR1 Signaling Pathway; ErbB1downstream signaling; HTLV-I infection; IL-3 Signaling Pathway; ImmuneSystem; Innate Immunity Signaling; MAP kinase activation in TLR cascade;MyD88 cascade initiated on plasma membrane; Myometrial Relaxation andContraction Pathways; NGF signalling via TRKA from the plasma membrane;Serotonin Receptor 4/6/7 and NR3C Signaling; Signalling by NGF; TRAF6Mediated Induction of proinflammatory cytokines; Toll Like Receptor 10(TLR10) Cascade; Toll Like Receptor 2 Cascade |
