||Recombinant human C9orf103protein, fused to His-tag at N-terminus, wasexpressed in E.coli and purified by conventional chromatography.
||C9orf103, also knownas probable gluconokinase, belongs to the gluconokinase gntK/gntV family.This protein is involved in carbohydrate acid metabolism and D-gluconatedegradation. Recombinant human C9orf103 protein, fused to His-tag atN-terminus, was expressed in E.coli and purified by using conventionalchromatography.
||Liquid. 20mMTris-HCl buffer (pH8.0) containing 20% glycerol 0.1 M NaCl, 1mM DTT.
||23.1 kDa (211aa),confirmed by MALDI-TOF.
||> 95% by SDS-PAGE
||0.5mg/ml (determinedby Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMAAPGA LLVMGVSGSG KSTVGALLAS ELGWKFYDAD DYHPEENRRKMGKGIPLNDQ DRIPWLCNLH DILLRDVASG QRVVLACSAL KKTYRDILTQ GKDGVALKCE ESGKEAKQAEMQLLVVHLSG SFEVISGRLL KREGHFMPPE LLQSQFETLE PPAAPENFIQ ISVDKNVSEI IATIMETLKMK
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.