||Recombinant human PPA1 protein, fused to His-tag at N-terminus,was expressed in E. coli and purified byusing conventional chromatography techniques.
||PPA1 belongsto the PPA1 family. This protein is an enzyme that catalyzesthe conversion of one molecule of pyrophosphate to two phosphate ions. PPA1 catalyzesthis hydrolysis reaction in the early steps of lipid degradation, a prominentexample of this phenomenon. By promoting the rapid hydrolysis ofpyrophosphate (PPi), PPA1 provides the driving force for the activation offatty acids destined for oxidation.
||Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200mM NaCl.
||35.2 kDa(313aa), confirmed by MALDI-TOF.
||> 95%by SDS-PAGE
||0.5mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMSGFST EERAAPFSLE YRVFLKNEKG QYISPFHDIP IYADKDVFHMVVEVPRWSNA KMEIATKDPL NPIKQDVKKG KLRYVANLFP YKGYIWNYGA IPQTWEDPGH NDKHTGCCGDNDPIDVCEIG SKVCARGEII GVKVLGILAM IDEGETDWKV IAINVDDPDA ANYNDINDVK RLKPGYLEATVDWFRRYKVP DGKPENEFAF NAEFKDKDFA IDIIKSTHDH WKALVTKKTN GKGISCMNTT LSESPFKCDPDAARAIVDAL PPPCESACTV PTDVDKWFHH QKN
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
|Pathways||CytosolictRNA aminoacylation; Gene Expression; Hypothetical Network for Drug Addiction;Oxidative phosphorylation; Oxidative phosphorylation|