"SSU72" Related Products

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Recombinant Human SSU72 RNA Polymerase II CTD Phosphatase Homolog (S. cerevisiae), His-tagged

Cat. No.: SSU72-1401H
Product Overview: Recombinanthuman SSU72protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventionalchromatography techniques.
Description: SSU72 is a highlyconserved homologue of yeast Ssu72, a CTD phosphatase and a component of thepolyadenylation/ termination machinery. Existing as multiple alternativelyspliced isoforms, SSU72 interacts with TFIIB, Rb and DNAM-1 and functions tocatalyze the dephosphorylation of target proteins, possibly playing a role inRNA processing and termination via dephosphorylation of Pol II.
Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl.
Molecular Weight: 25 kDa (217aa),confirmed by MALDI-TOF.
Purity: > 95%by SDS-PAGE
Concentration: 1 mg/ml(determined by Bradford assay)
Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSMPSSPLR VAVVCSSNQN RSMEAHNILS KRGFSVRSFG TGTHVKLPGPAPDKPNVYDF KTTYDQMYND LLRKDKELYT QNGILHMLDR NKRIKPRPERFQNCKDLFDL ILTCEERVYD QVVEDLNSRE QETCQPVHVV NVDIQDNHEE ATLGAFLICE LCQCIQHTEDMENEIDELLQ EFEEKSGRTF LHTVCFY
Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
nullValue_other:
Functionhydrolaseactivity; phosphoproteinphosphatase activity
Gene Name: SSU72SSU72 RNA polymerase II CTD phosphatase homolog (S. cerevisiae) [ Homosapiens ]
Official Symbol: SSU72
Synonyms: SSU72; SSU72 RNA polymerase II CTD phosphatase homolog(S. cerevisiae); HSPC182; FLJ13048; PNAS-120; RNA polymerase II subunit A C-terminal domainphosphatase SSU72; CTD phosphatase SSU72; OTTHUMP00000000597; EC3.1.3.16; Ssu72 RNA polymerase IICTD phosphatase homolog (yeast); OTTHUMP00000000598; OTTHUMP00000000599;OTTHUMP00000039178
Gene ID: 29101
mRNA Refseq: NM_014188
Protein Refseq: NP_054907
UniProt ID: Q9NP77
Chromosome Location: 1p36.3

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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