Recombinant Human Tumor Necrosis Factor, HQ-tagged

Cat. No.: TNF-1914H
Product Overview: Recombinant humanTNF-alpha protein contains 158 amino acid residues and a 16 aa HQ tag. Totallength is 174 aa.
Description: Tumor necrosis factor-alphais the best-known member of this class, and sometimes referred to when theterm tumor necrosis factor is used. TNF is a monocyte-derived cytotoxin thathas been implicated in tumour regression, septic shock and cachexia.
Source: Hordeum vulgare (barleygrain). Barley grain"s proteolytic activity is almost 50 times less than E.coli or mammalian cells. Barley seed has no human or animal viralcontaminants, which is ideal for cell culture and in vitro and in vivobiological experiments.
Species: Human
Form: Lyophilized from a0.2 μm sterile filtered solution of PBS, pH 7.2.
Purity: >98% by SDS-PAGE.Purified product carries no pyrogenic or pro-inflammatory contaminants, asassayed with monocyte activation test using custom human Multiplex CytokineAssay measuring IL-6, TNF-alpha and IL-1beta induction.
Molecular Weight: Projected MW 19.6kDa. The recombinant protein migrates as two bands with an apparent MW 21 and23 kDa in SDS-PAGE.
Endotoxin Level: < 0.005 ng per μgof product (0.05 EU/μg) as measured by turbidimetric kinetic assay.
Reconstitution: Centrifuge vialprior to opening. First add sterile distilled water to the vial to fullysolubilize the protein to a concentration not less than 100 μg/ml. Aftercomplete solubilization of the protein, it can be further diluted to otheraqueous solutions.
Biological Activity: The ED50is determined by the Cytotoxicity Assay for the human-TNF-alpha fordosedependent cytolytic effects of serial dilutions of h-TNF-alpha on thefibrosarcoma L-929 cells. The ED50 for a typical batch ofrecombinant human TNF-alpha is 40 ng/ml.
Storage: The lyophilizedprotein, though stable at room temperature for two weeks, is best stored at-20°C. Reconstituted protein should be used immediately or stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles.
OfficialSymbol: TNF
Pathways: Adipocytokinesignaling pathway; Adipogenesis; African trypanosomiasis; Allograftrejection; Alzheimer" s disease; Amoebiasis; Amyotrophic lateral sclerosis(ALS); Angiopoietin receptor Tie2-mediated signaling; Antigen processing andpresentation; Apoptosis; Asthma; Calcineurin-regulated NFAT-dependenttranscription in lymphocytes; Canonical NF-kappaB pathway; Caspase cascade inapoptosis; Cellular roles of Anthrax toxin; Ceramide signaling pathway
Gene Name: TNF tumor necrosis factor [ Homosapiens ]
Synonyms: TNF;tumor necrosis factor; DIF; TNFA; TNFSF2; TNF-alpha; TNF-a; cachectin;APC1 protein; OTTHUMP00000029281; TNF, monocyte-derived; TNF,macrophage-derived; TNF superfamily, member 2; tumor necrosis factor alpha;tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member2; Tumornecrosis factor; Cachectin
Gene ID: 7124
mRNA Refseq: NM_000594
Protein Refseq: NP_000585
MIM: 191160
UniProt ID: P01375
Chromosome Location: 6p21.3
Function: cytokine activity; identicalprotein binding; protease binding; protein binding; transcription regulatoryregion DNA binding; tumor necrosis factor receptor binding

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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