| Cat. No. : |
TNF-1914H |
| Description : |
Tumor necrosis factor-alphais the best-known member of this class, and sometimes referred to when theterm tumor necrosis factor is used. TNF is a monocyte-derived cytotoxin thathas been implicated in tumour regression, septic shock and cachexia. |
| Source : |
Hordeum vulgare (barleygrain). Barley grain"s proteolytic activity is almost 50 times less than E.coli or mammalian cells. Barley seed has no human or animal viralcontaminants, which is ideal for cell culture and in vitro and in vivobiological experiments. |
| Species : |
Human |
| Form : |
Lyophilized from a0.2 μm sterile filtered solution of PBS, pH 7.2. |
| Purity : |
>98% by SDS-PAGE.Purified product carries no pyrogenic or pro-inflammatory contaminants, asassayed with monocyte activation test using custom human Multiplex CytokineAssay measuring IL-6, TNF-alpha and IL-1beta induction. |
| Molecular Weight : |
Projected MW 19.6kDa. The recombinant protein migrates as two bands with an apparent MW 21 and23 kDa in SDS-PAGE. |
| Endotoxin Level : |
< 0.005 ng per μgof product (0.05 EU/μg) as measured by turbidimetric kinetic assay. |
| Reconstitution : |
Centrifuge vialprior to opening. First add sterile distilled water to the vial to fullysolubilize the protein to a concentration not less than 100 μg/ml. Aftercomplete solubilization of the protein, it can be further diluted to otheraqueous solutions. |
| Biological Activity : |
The ED50is determined by the Cytotoxicity Assay for the human-TNF-alpha fordosedependent cytolytic effects of serial dilutions of h-TNF-alpha on thefibrosarcoma L-929 cells. The ED50 for a typical batch ofrecombinant human TNF-alpha is 40 ng/ml. |
| Storage : |
The lyophilizedprotein, though stable at room temperature for two weeks, is best stored at-20°C. Reconstituted protein should be used immediately or stored in workingaliquots at -20°C. Avoid repeated freeze-thaw cycles. |
| OfficialSymbol : |
TNF |
| Pathways : |
Adipocytokinesignaling pathway; Adipogenesis; African trypanosomiasis; Allograftrejection; Alzheimer" s disease; Amoebiasis; Amyotrophic lateral sclerosis(ALS); Angiopoietin receptor Tie2-mediated signaling; Antigen processing andpresentation; Apoptosis; Asthma; Calcineurin-regulated NFAT-dependenttranscription in lymphocytes; Canonical NF-kappaB pathway; Caspase cascade inapoptosis; Cellular roles of Anthrax toxin; Ceramide signaling pathway |
