||Recombinanthuman full length LYN with C-terminal His tag. LYN is a SRC family kinaseexpressed predominantly in hematopoietic cells.
||LYN is a member ofthe Src family of protein tyrosine kinases, which is mainly expressed inhematopoietic cells, in neural tissues liver, and adipose tissue. In varioushematopoietic cells, LYN has emerged as a key enzyme involved inthe regulation of cell activation. In these cells, a small amount of LYN isassociated with cell surface receptor proteins, including the B cell antigenreceptor (BCR), CD40, or CD19.
||Liquid in50 mM Tris, pH 7.5 + 0.05 mM EDTA + 1 mM DTT + 100 mM NaCl + 0.05% NP-40 +50% glycerol.
||> 95.0% asdetermined by RP-HPLC and SDS-PAGE analyses.
||772 nmoleof phosphate transferred to poly [Glu, Tyr] 4:1 substrate per minute per mgof totalprotein at 30°C.Activity determined at a final protein concentration of 2 μg/ml.
||Stablefor 6 months in working aliquots at -80°C. Avoidrepeated freeze-thaw cycles.
|Function||ubiquitin proteinligase binding; receptor signaling protein tyrosine kinase activity; proteintyrosine kinase activity; protein binding; platelet-derived growth factor receptorbinding; phosphoprotein binding; nucleotide binding; non-membrane spanningprotein tyrosine kinase activity; ion channel binding; integrin binding; glycosphingolipidbinding; SH3 domain binding; ATP binding|