"Hip1" Related Products

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Recombinant Mouse Huntingtin Interacting Protein 1, His-tagged

Cat. No.: Hip1-5638M
Product Overview: RecombinantMouse Hip1 protein was expressed in Murine myeloma cell line, with theMouse Hip Lys24-Arg678 & Asp52-Arg678 (N-terminus) and aC-terminal 10-His tag.
Description: Hedgehogsignaling proteins act as mitogens, morphogens, or inducing factors in manydifferent cell types during embryonic development. They aid in growth, patterning,and morphogenesis in both vertebrates and insects. Hip1 (Hedgehog-interactingprotein) is a type I transmembrane protein identified for its ability to bindbiologically active Sonic Hedgehog. It is comprised of 700 aa, and includes ahydrophobic signal sequence, two EGF-like domains near the C-terminus, and a22 amino acid membrane-spanning region at the C-terminal end. Hip1 has only beenidentified in vertebrates and binds all three mammalian Hedgehogs: sonic(Shh),desert (Dhh), and Indian (Ihh).
Source: Murine myeloma cellline, NS0-derived.
Form: Lyophilized from a0.2 um filtered solution in PBS with BSA as a carrier protein.
N-terminalSequence: Lys24 & Asp52
MolecularWeight: 76 kDa & 73 kDa
Activity: Measuredby its ability to inhibit Sonic Hedgehog (Shh) induction of alkalinephosphatase production in C3H10T1/2 mouse fibroblasts. The ED50 for thiseffect is typically 1.5-7.5 μg/mL in the presence of 5 μg/mL rmShh.
Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
SDS-PAGE: 80-90kDa and 58-64 kDa, reducing conditions.
Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution: 100μg/mL in sterile PBS
Storage: Use a manual defrostfreezer and avoid repeated freeze-thaw cycles. 12 months from dateof receipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.
Gene Name: Hip1 huntingtin interactingprotein 1 [ Mus musculus ]
Official Symbol: Hip1
Synonyms: Hip1; huntingtininteracting protein 1; mKIAA4113;2610109B09Rik; A930014B11Rik; E130315I21Rik; KIAA4113; MGC27616; HIP-I; huntingtin-interactingprotein I
Gene ID: 215114
mRNA Refseq: NM_146001
Protein Refseq: NP_666113
UniProt ID: Q8C303
Chromosome Location: 5
Pathway: EGFR1 SignalingPathway; Huntington"s disease
Function: actin binding; binding;clathrin binding; phosphatidylinositol binding; phospholipid binding; proteinbinding

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