||Recombinant human CAPSL protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
||CAPSL, also known as Calcyphosine-like protein, is a calcium-binding protein containing two calcium-binding motifs (EF-hands). These conserved domains are found in a superfamily of calcium sensors and calcium signal modulators. Furthermore, the CAPSL gene is in the same linkage disequilibrium (LD) block as the IL7R gene. It was reported association between the CAPSL-IL7R locus and type 1 diabetes.
||Liquid. In 20 mMTris-HCl buffer (pH8.0) containing 0.2M NaCl,5mM DTT, 20% glycerol
||26.3 kDa (228aa), confirmed by MALDI-TOF
||> 90% by SDS - PAGE
||1 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MAGTARHDRE MAIQAKKKLT TATDPIERLR LQCLARGSAG IKGLGRVFRI MDDDNNRTLD FKEFMKGLND YAVVMEKEEV EELFQRFDKD GNGTIDFNEF LLTLRPPMSR ARKEVIMQAF RKLDKTGDGV ITIEDLREVY NAKHHPKYQN GEWSEEQVFR KFLDNFDSPY DKDGLVTPEE FMNYYAGVSA SIDTDVYFII MMRTAWKL
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.