Active Recombinant Human Angiogenic Factor With G Patch And FHA Domains 1, His-tagged

• Cat.No.: AGGF1-543H
• Product Overview: Recombinant Human AGGF1protein was expressed in Murine myeloma cell line with a N-terminal 9-His tag.Ala2-Glu714.

Specification

• Species: Human
• Source: Mammalian Cells
• Tag: His
• Protein Length: 2-714 a.a.
• Description: AGGF1, wasidentified by its association with KlippelTrenaunay syndrome (KTS), acongenital vascular morphogenesis disorder. Two genetic alterations found inKTS lead to aberrant expression or function of AGGF1: a chromosomal translocationthat falls within the AGGF1 promoter and a glutamate to lysine point mutationat position 133. The human AGGF1 cDNA encodes a 714 amino acid (aa) precursorwith a coiled coil domain, an FHA domain, a G-patch domain, and an OCREdomain. FHA domains bind phosphorylated threonine and tyrosine residues.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: His
• Molecular Weight: 82.0 kDa
• Activity: Measured by itsbinding ability in a functional ELISA. Immobilized rh AGGF1 at 1 μg/mL (100μL/well) can bind rhTWEAK with a linear range of 0.8-50 ng/mL.
• Endotoxin Level: < 1.0EU per 1 μg of the protein by the LAL method.
• SDS-PAGE: 120-140 kDa,reducing conditions.
• Purity: > 95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100μg/mL in sterile PBS.
• Storage: Use a manualdefrost freezer and avoid repeated freeze-thaw cycles. 12 months from date ofreceipt, -20 to -70°C as supplied. 1 month, 2 to 8°C under sterile conditionsafter reconstitution. 3 months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: AGGF1 angiogenic factor with Gpatch and FHA domains 1 [ Homo sapiens ]
• Official Symbol: AGGF1
• Synonyms: AGGF1; angiogenicfactor with G patch and FHA domains 1; VG5Q; GPATC7;GPATCH7; HSU84971; HUS84971; angiogenic factor VG5Q; vasculogenesis gene on5q protein; patch domain-containing protein 7; G5Q
• Gene ID: 55109
• mRNA Refseq: NM_018046
• Protein Refseq: NP_060516
• MIM: 608464
• UniProt ID: Q8N302
• Chromosome Location: 5q13.3
• Function: eukaryotic cellsurface binding; nucleic acid binding; protein binding