Recombinant E.coli Map, His-tagged

• Cat.No.: map-979E
• Product Overview: Recombinant E.colimap protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques(1-264aa).

Specification

• Species: E.coli
• Source: E.coli
• Tag: His
• Description: Methionine aminopeptidases, also designated peptidase M proteins, are members of the M24 family of proteins. This protein removes the amino-terminal methionine residue from nascent polypeptides. The active site of map contains two adjacent divalent metal ions connected by a water molecule or hydroxide ion.
• Form: Liquid. In 20mM Tris-HClbuffer(pH 8.0) containing 10% glycerol,2mM DTT.
• Molecular Weight: 31.5 kDa (284aa) confirmed by MALDI-TOF
• Purity: > 90% by SDS - PAGE
• Concentration: 1 mg/ml (determined by Bradford assay)
• Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MAISIKTPED IEKMRVAGRL AAEVLEMIEP YVKPGVSTGE LDRICNDYIV NEQHAVSACL GYHGYPKSVC ISINEVVCHG IPDDAKLLKD GDIVNIDVTV IKDGFHGDTS KMFIVGKPTI MGERLCRITQ ESLYLALRMV KPGINLREIG AAIQKFVEAE GFSVVREYCG HGIGRGFHEE PQVLHYDSRE TNVVLKPGMT FTIEPMVNAG KKEIRTMKDG WTVKTKDRSL SAQYEHTIVV TDNGCEILTL RKDDTIPAII SHDE
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: map methionine aminopeptidase [ Escherichia coli str. K-12 substr. MG1655 ]
• Synonyms: map; ECK0166; JW0163; pepM; methionine aminopeptidase; NP_414710.1; EC 3.4.11.18
• Gene ID: 947882
• Protein Refseq: NP_414710