||Recombinant E. coli msrB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques(1-137aa).
||MsrB, also known as, methionine sulfoxide reductase B from Escherichia coli, belongs to the msrB Met sulfoxide reductase family. E.coli msrB carries out the reduction of methionine-R-sulfoxide to methionine. This protein possess a metal binding site composed of two CXXC motifs. The bound metal (zinc or iron) may stabilize the conformation of the enzymes.
||Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol; 0.1M NaCl,1mM DTT
||17.6 kDa (157aa) confirmed by MALDI-TOF
||> 95% by SDS - PAGE
||1.0 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MANKPSAEEL KKNLSEMQFY VTQNHGTEPP FTGRLLHNKR DGVYHCLICD APLFHSQTKY DSGCGWPSFYEPVSEESIRY IKDLSHGMQR IEIRCGNCDA HLGHVFPDGP QPTGERYCVN SASLRFTDGE NGEEING
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.