"msrB" Related Products

Recombinant E.coli MsrB, His-tagged

Cat. No.: msrB-980E
Product Overview: Recombinant E. coli msrB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques(1-137aa).
Description: MsrB, also known as, methionine sulfoxide reductase B from Escherichia coli, belongs to the msrB Met sulfoxide reductase family. E.coli msrB carries out the reduction of methionine-R-sulfoxide to methionine. This protein possess a metal binding site composed of two CXXC motifs. The bound metal (zinc or iron) may stabilize the conformation of the enzymes.
Form: Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol; 0.1M NaCl,1mM DTT
Molecular Weight: 17.6 kDa (157aa) confirmed by MALDI-TOF
Purity: > 95% by SDS - PAGE
Concentration: 1.0 mg/ml (determined by Bradford assay)
Sequences of amino acids: MGSSHHHHHH SSGLVPRGSH MANKPSAEEL KKNLSEMQFY VTQNHGTEPP FTGRLLHNKR DGVYHCLICD APLFHSQTKY DSGCGWPSFYEPVSEESIRY IKDLSHGMQR IEIRCGNCDA HLGHVFPDGP QPTGERYCVN SASLRFTDGE NGEEING
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name: msrB methionine sulfoxide reductase B [ Escherichia coli str. K-12 substr. MG1655 ]
Synonyms: msrB; methionine sulfoxide reductase B; ECK1776; JW1767; EC 1.8.4.12
Gene ID: 947188
Protein Refseq: NP_416292

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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