||Recombinant humanBLOC1S2 protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques
||BLOC1S2 (Biogenesisof lysosome-related organelles complex 1 subunit 2) belongs to the BLOC1S2family. BLOC-1 or biogenesis of lysosome-related organelles complex 1 is aubiquitously expressed multisubunit protein complex. BLOC-1 is required fornormal biogenesis of specialized organelles of the endosomal-lysosomalsystem, such as melanosomes and platelet dense granules. This protein plays arole in cell proliferation.
||Liquid. In 20 mM Tris-HClbuffer, pH8.0, 10% glycerol, 1mM DTT, 50mM NaCl
||18.5kDa (166aa),confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
||> 85% by SDS -PAGE
||1mg/ml (determinedby Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHHSSGLVPRGSH MGSHMAAAAE GVLATRSDEP ARDDAAVETA EEAKEPAEAD ITELCRDMFS KMATYLTGELTATSEDYKLL ENMNKLTSLK YLEMKDIAIN ISRNLKDLNQ KYAGLQPYLD QINVIEEQVA ALEQAAYKLDAYSKKLEAKY KKLEKR
||Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles.