Recombinant Human Clathrin, Light Chain A, His-tagged

• Cat.No.: CLTA-6787H
• Product Overview: Recombinant humanCLTA protein, fused to His-tag at N-terminus, was expressed in E.coli andpurified by using conventional chromatography techniques

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: Clathrin, lightchain A, also known as CLTA, belongs to the clathrin family. Clathrin is alarge, soluble protein composed of heavy and light chains. It is a majorcytosolic coat protein in pits and vesicles originating from the plasmamembrane and the trans-Golgi network. In receptor-mediated endocytosis,receptor proteins are captured by Clathrin-coated vesicles. CLTA is one oftwo clathrin light chain proteins which are believed to function asregulatory elements
• Form: Liquid. In 20mMTris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol
• Molecular Weight: 26.2 kDa (242aa),confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• Purity: > 90% by SDS -PAGE
• Concentration: 1 mg/ml (determinedby Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMAELDP FGAPAGAPGG PALGNGVAGA GEEDPAAAFL AQQESEIAGI ENDEAFAILDGGAPGPQPHGEPPGGPDAVDGVMNGEYYQE SNGPTDSYAA ISQVDRLQSE PESIRKWREE QMERLEALDA NSRKQEAEWK EKAIKELEEWYARQDEQLQKTKANNRAAEEAFVNDIDESS PGTEWERVAR LCDFNPKSSK QAKDVSRMRS VLISLKQAPL VH
• Storage: Can be stored at+4°C short term (1-2 weeks). For long term storage, aliquot and store at-20°C or -70°C. Avoid repeated freezing and thawing cycles
• Officialsymbol: CLTA

Gene Information

• Gene Name: CLTA clathrin, light chain A[Homo sapiens]
• Synonyms: clathrin,light chain A; OTTHUMP00000215626; clathrin, light polypeptide (Lca);OTTHUMP00000215628; Lca; clathrin light chain A; OTTHUMP00000021365;OTTHUMP00000021366; OTTHUMP00000215627
• Gene ID: 1211
• mRNA Refseq: NM_001833
• Protein Refseq: NP_001824
• MIM: 118960
• UniProt ID: P09496
• Chromosome Location: 9p13
• Pathway: Arf1pathway, organism-specific biosystem; Axon guidance, organism-specificbiosystem; Bacterial invasion of epithelial cells, organism-specificbiosystem; Bacterial invasion of epithelial cells, conserved biosystem; C-MYBtranscription factor network, organism-specific biosystem; Clathrin derivedvesicle budding, organism-specific biosystem
• Function: molecular_function;peptide binding; structural molecule activity