Recombinant E.coli NanA, His-tagged
Cat.No. : | nanA-983E |
Product Overview : | Recombinant E.colinanA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography(1-297aa). |
- Specification
- Gene Information
- Related Products
Cat. No. : | nanA-983E |
Description : | NanA, also known as N-acetylneuraminatelyase, belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. NanA catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetyl-D-mannosamine. This protein was inhibited by reduction with NaBH4 in the presence of the substrate, indicating that it belongs to the Schiff-base-forming Class Ialdolases. NanA was strongly inhibited by Cu2+ ions, p-chloromercuribenzoate and N-bromosuccinimide, and also inhibited competitively by the reaction product, pyruvate, and its structurally related compounds, dihydroxyacetone and DL-glyceraldehyde. |
Form : | Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol |
Molecular Weight : | 34.7 kDa (317aa) confirmed by MALDI-TOF |
Purity : | > 95% by SDS - PAGE |
Concentration : | 1 mg/ml (determined by Bradford assay) |
Sequences of amino acids : | MGSSHHHHHH SSGLVPRGSH MATNLRGVMA ALLTPFDQQQ ALDKASLRRL VQFNIQQGID GLYVGGSTGE AFVQSLSERE QVLEIVAEEAKGKIKLIAHV GCVSTAESQQ LAASAKRYGF DAVSAVTPFY YPFSFEEHCD HYRAIIDSAD GLPMVVYNIP ALSGVKLTLD QINTLVTLPGVGALKQTSGD LYQMEQIRRE HPDLVLYNGY DEIFASGLLA GADGGIGSTY NIMGWRYQGI VKALKEGDIQ TAQKLQTECN KVIDLLIKTGVFRGLKTVLH YMDVVSVPLC RKPFGPVDEK YLPELKALAQ QLMQERG |
Storage : | Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
Gene Name : | nanA N-acetylneuraminate lyase [ Escherichia coli str. K-12 substr. MG1655 ] |
Synonyms : | nanA, ECK3214; JW3194; npl; N-acetylneuraminatelyase; NP_417692.1; EC 4.1.3.3; N-acetylneuraminatelyase (aldolase); catabolism of sialic acid; not K-12? |
Gene ID : | 947742 |
Protein Refseq : | NP_417692 |
Products Types
◆ Recombinant Protein | ||
nanA-1299E | Recombinant E. coli (strain K12) nanA Protein (Full Length), C-His tagged | +Inquiry |
nanA-1302E | Recombinant E. coli (strain K12/DH10B) nanA Protein (Met1-Gly297), C-His and Strep tagged | +Inquiry |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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