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Recombinant E.coli CysH, His-tagged

Cat.No. : cysH-987E
Product Overview : Recombinant E.coli cysH protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques(1-244aa).
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  • Gene Information
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Cat. No. : cysH-987E
Description : CysH (Phosphoadenosine phosphosulfate reductase) belongs to the PAPS reductase family, specifically those acting on a sulfur group of donors with a disulfide as acceptor. In enzymology, a cysH is an enzyme that catalyzes the chemical reaction. Three substrates of this enzyme are adenosine 3",5"-bisphosphate, sulfite, and thioredoxin disulfide, whereas its two products are 3"-phosphoadenylyl sulfate and thioredoxin.
Form : Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl
Molecular Weight : 30.1kDa (264aa) confirmed by MALDI-TOF
Purity : > 90% by SDS - PAGE
Concentration : 0.5mg/ml (determined by Bradford assay)
Sequences of amino acids : MGSSHHHHHH SSGLVPRGSH MSKLDLNALN ELPKVDRILA LAETNAELEK LDAEGRVAWA LDNLPGEYVL SSSFGIQAAV SLHLVNQIRP DIPVILTDTG YLFPETYRFI DELTDKLKLN LKVYRATESA AWQEARYGKL WEQGVEGIEK YNDINKVEPM NRALKELNAQ TWFAGLRREQ SGSRANLPVL AIQRGVFKVL PIIDWDNRTI YQYLQKHGLK YHPLWDEGYL SVGDTHTTRK WEPGMAEEET RFFGLKRECG LHEG
Storage : Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.
Gene Name : cysH 3"-phosphoadenosine 5"-phosphosulfate reductase [ Escherichia coli str. K-12 substr. MG1655 ]
Synonyms : cysH; ECK2757; JW2732; 3"-phosphoadenosine 5"-phosphosulfate reductase; NP_417242.1; EC 1.8.4.8
Gene ID : 947230
Protein Refseq : NP_417242

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