||Recombinant human CABP7 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography (1-188aa).
||CABP7, also known as Calcium-binding protein 7, contains two EF-hand domains. It negatively regulates Golgi-to-plasma membrane trafficking by interacting with PI4KB and inhibiting its activity. The CaBP family of EF-hand containing small Ca (2+)-binding proteins has recently emerged as important regulators of multiple targets essential to normal neuronal function in the mammalian central nervous system.
||Liquid. Phosphate-buffered saline (pH 7.4)
||23.7 kDa (208aa) confirmed by MALDI-TOF
||> 80% by SDS - PAGE
||0.5 mg/ml (determined by Bradford assay)
|Sequences of amino acids:
||MGSSHHHHHH SSGLVPRGSH MPFHPVTAAL MYRGIYTVPN LLSEQRPVDI PEDELEEIRE AFKVFDRDGN GFISKQELGT AMRSLGYMPN EVELEVIIQR LDMDGDGQVD FEEFVTLLGP KLSTSGIPEK FHGTDFDTVF WKCDMQKLTV DELKRLLYDT FCEHLSMKDI ENIIMTEEES HLGTAEECPV DVETCSNQQI RQTCVRKS
||Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.