||RecombinantHuman ALDH4A1 protein was expressed as GST-tagged fusion proteins by E. coli andpurified by GSH-sepharose. The purified ALDH4A1 was resolved in 1M PBS (58mMNa2HPO4, 17mM NaH2PO4, 68mM NaCl,pH8) added with 100mM GSH and 1% Triton X-100, 15% glycerol.
||Delta-1-pyrroline-5-carboxylatedehydrogenase, mitochondrial is an enzyme that in humans is encoded by theALDH4A1 gene. This protein belongs to the aldehyde dehydrogenase family ofproteins. This enzyme is a mitochondrial matrix NAD-dependent dehydrogenasethat catalyzes the second step of the proline degradation pathway, convertingpyrroline-5-carboxylate to glutamate. Deficiency of this enzyme is associatedwith type II hyperprolinemia, an autosomal recessive disorder characterizedby accumulation of delta-1-pyrroline-5-carboxylate (P5C) and proline. Twotranscript variants encoding the same protein have been identified for thisgene.
||1MPBS (58mM Na2HPO4, 17mM NaH2PO4,68mM NaCl, pH8) added with 100mM GSH and 1% Triton X-100, 15% glycerol.
||Theprotein is stored in PBS at -20°C. Repeatedfreeze-thaw cycles should be avoided.