||Recombinant humanCDC26 protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography.
||CDC26,also known as ANAPC12, APC12, belongs to the CDC26 family which orchestrates progressionthrough mitosis by decorating cell-cycle regulators with ubiquitin chains.This protein is component of the anaphase promoting complex/cyclosome (APC/C),a cell cycle-regulated E3 ubiquitin ligase that controls progression throughmitosis and the G1 phase of the cell cycle. The APC/C complex acts bymediating ubiquitination and subsequent degradation of target proteins.
||Liquid. 20mM Tris-HClbuffer (pH8.0) containing 40% glycerol, 0.1M NaCl.
||11.9 kDa (105 aa), confirmed by MALDI-TOF.
||> 90%by SDS - PAGE
||1 mg/ml(determined by Bradford assay)
|Sequences of aminoacids:
||MGSSHHHHHH SSGLVPRGSH MLRRKPTRLELK LDDIEEFE NIRKDLETRK KQKEDVEVVG GSDGEGAIGL SSDPKSREQM INDRIGYKPQ PKPNNRSSQFGSLEF
||Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.